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B. Sc. Biochemistry & Molecular Biology
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#DNA
Distinguish between cis and trans regulation.
[
BIOC 4404
]
Interpret plasmid maintenance and evolution in terms of plasmid genes (poison-antidote mechanism).
[
BIOC 4403
]
Summarize in writing research done on a recombinant DNA-related topic.
[
BIOC 4501
]
Compare mobile genetic elements (DNA transposons, retrotransposons) as drivers of genome evolution.
[
BIOC 4403
]
Create and give an oral presentation based on independent research of a recombinant DNA-related topic.
[
BIOC 4501
]
Interpret roles of DNA methylation in genome evolution (CpG islands) and gene regulation (epigenetics).
[
BIOC 4403
]
Recall DNA replication, transcription, and RNA processing.
[
BIOC 4403
]
Describe the structural features of nucleic acids and be able to distinguish them; describe the basis for information content in a DNA sequence; describe the flow of genetic information and be aware of different discoveries emerging in this area.
[
BIOC 2300
]
Distinguish the nucleotides that compose DNA and RNA.
[
BIOC 3400
]
Given DNA sequence data design (probes), recognize their use in different techniques such as PCR, microarray, southern blotting.
[
BIOC 3400
]
Apply/use knowledge on DNA replication to explain the behavior of these molecules in different stages and under specific conditions (UV, analogs, salt concentration).
[
BIOC 3400
]
Distinguish between RNA dependent and DNA dependent Polymerases and identify the process where they are involved.
[
BIOC 3400
]
Explain the molecular basis of procedures such as gene cloning, cDNA and genomic DNA library screening, polymerase chain reaction (PCR) amplification of DNA, DNA sequencing, microarray technology, nuclear transfer somatic cell and induced pluripotent stem cell cloning, gene therapy.
[
BIOC 4501
]
Explain the process of chain termination DNA sequencing.
[
BIOC 3400
]
Given a DNA sequence and restriction enzyme data, analyze a band pattern in a gel and generate a restriction map.
[
BIOC 3400
]
Design a strategy for manipulating DNA molecules in particular scenarios (eg. Radio labeling and sub cloning).
[
BIOC 3400
]
Distinguish the differences between prokaryotes and eukaryotes in terms of genome organization, gene structure, and expression and DNA replication.
[
BIOC 3400
]
Given a type of DNA damage explain the repair mechanisms that can occur.
[
BIOC 3400
]
Identify/recognize and explain the roles of the major proteins involved in the process of DNA replication/RNA synthesis and its regulation.
[
BIOC 3400
]
Compare different techniques of next generation DNA sequencing in terms of advantages/disadvantages and fields of application.
[
BIOC 4403
]
Distinguish the differences in DNA packaging/chromatin organization between prokaryotes and eukaryotes.
[
BIOC 3400
]
Identify the different types of DNA and RNA sequence elements regulating eukaryotic gene expression.
[
BIOC 4404
]
Predict the mRNA and the amino acid sequence given a DNA sequence.
[
BIOC 3400
]
Explain how different types of DNA and RNA sequence elements are involved regulating eukaryotic gene expression.
[
BIOC 4404
]
Explain the contribution of DNA packaging/chromatin to gene expression and regulation.
[
BIOC 3400
]
Identify techniques to study DNA packaging/chromatin (eg CHIP, DNA sensitivity).
[
BIOC 3400
]
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