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B. Sc. Biochemistry & Molecular Biology
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#gene
Compare representative mitochondrial genomes (human, plants, algae) in terms of gene content, organization, and expression.
[
BIOC 4403
]
Describe the basic principles of intracellular signal transduction and discuss how these processes may alter gene expression, protein function and cellular fate.
[
multiple courses
]
Distinguish between cis and trans regulation.
[
BIOC 4404
]
Distinguish between gene activation and gene repression.
[
BIOC 4404
]
Identify and distinguish intervening sequences (group I introns, group II introns, twintrons, split intron, inteins), using key structural and mechanistic features.
[
BIOC 4403
]
Identify key evidences/arguments for hypothesis on the origin/evolution of genes from an RNA world.
[
BIOC 4403
]
Interpret features of bacterial genome organization: non-random positioning and orientation of certain genes in relationship to overlapping rounds of genome replication.
[
BIOC 4403
]
Interpret plasmid maintenance and evolution in terms of plasmid genes (poison-antidote mechanism).
[
BIOC 4403
]
Specify methods to investigate gene regulation.
[
BIOC 4404
]
Identify the different types of protein factors regulating bacterial gene expression.
[
BIOC 4404
]
Interpret genetic mobility of introns and inteins, by comparing mechanisms of intron/intein homing and transposition.
[
BIOC 4403
]
Recognize different types of non-coding RNAs, and recognize/identify their roles in gene expression.
[
BIOC 3400
]
Classify different regulatory mechanisms of gene transcription.
[
BIOC 4404
]
Identify steps and mechanisms of evolutionary gene transfer from organelle genomes to nuclear genome.
[
BIOC 4403
]
Explain the molecular basis of procedures such as gene cloning, cDNA and genomic DNA library screening, polymerase chain reaction (PCR) amplification of DNA, DNA sequencing, microarray technology, nuclear transfer somatic cell and induced pluripotent stem cell cloning, gene therapy.
[
BIOC 4501
]
Design oligonucleotides probes and primers for molecular biology applications including manipulation of genes, PCR and other hybridization-based approaches.
[
BIOC 4501
]
Distinguish the differences between prokaryotes and eukaryotes in terms of genome organization, gene structure, and expression and DNA replication.
[
BIOC 3400
]
Identify the effects of mRNA localization on the consequences and regulation of gene expression.
[
BIOC 4404
]
Identify the different types of DNA and RNA sequence elements regulating eukaryotic gene expression.
[
BIOC 4404
]
Identify the different types of protein factors regulating eukaryotic gene expression.
[
BIOC 4404
]
Explain how different types of DNA and RNA sequence elements are involved regulating eukaryotic gene expression.
[
BIOC 4404
]
Explain the contribution of DNA packaging/chromatin to gene expression and regulation.
[
BIOC 3400
]
Describe the challenges and opportunities for gene regulation offered by chromatin structure.
[
BIOC 4404
]
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