Recognize different types of non-coding RNAs, and recognize/identify their roles in gene expression.Explain the steps of protein synthesis identifying the differences between prokaryotes and eukaryotes.Distinguish the nucleotides that compose DNA and RNA.Given DNA sequence data design (probes), recognize their use in different techniques such as PCR, microarray, southern blotting.Predict the fate of a protein based on features of the primary sequence.Apply/use knowledge on DNA replication to explain the behavior of these molecules in different stages and under specific conditions (UV, analogs, salt concentration).Distinguish between RNA dependent and DNA dependent Polymerases and identify the process where they are involved.Explain the process of chain termination DNA sequencing.Explain the processing (splicing, editing) of RNA (mRNA, tRNA, rRNA). Predict the effects of mutations in the primary RNA sequence.Given a DNA sequence and restriction enzyme data, analyze a band pattern in a gel and generate a restriction map.Identify the specific nucleotide and recognize the distinguishing features.Interpret data obtained from electrophoresis based methods (eg blotting, restriction enzyme, PFGE, gel mobility assays and CHIP).Based on the properties of the polynucleotides predict the outcome of differential chemical or enzymatic treatment.Design a strategy for manipulating DNA molecules in particular scenarios (eg. Radio labeling and sub cloning).Distinguish the differences between prokaryotes and eukaryotes in terms of genome organization, gene structure, and expression and DNA replication.Given a type of DNA damage explain the repair mechanisms that can occur.Identify/recognize and explain the roles of the major proteins involved in the process of DNA replication/RNA synthesis and its regulation.Distinguish the differences in DNA packaging/chromatin organization between prokaryotes and eukaryotes.Identify the RNA molecules that participate protein synthesis and explain their contributions.Predict the mRNA and the amino acid sequence given a DNA sequence.Explain the contribution of DNA packaging/chromatin to gene expression and regulation.Provide evidence for the roles of catalytic RNA in different cellular process such as protein synthesis and RNA splicingIdentify techniques to study DNA packaging/chromatin (eg CHIP, DNA sensitivity).