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B. Sc. Biochemistry & Molecular Biology
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#DNA
Distinguish the nucleotides that compose DNA and RNA.
Given DNA sequence data design (probes), recognize their use in different techniques such as PCR, microarray, southern blotting.
Apply/use knowledge on DNA replication to explain the behavior of these molecules in different stages and under specific conditions (UV, analogs, salt concentration).
Distinguish between RNA dependent and DNA dependent Polymerases and identify the process where they are involved.
Explain the process of chain termination DNA sequencing.
Given a DNA sequence and restriction enzyme data, analyze a band pattern in a gel and generate a restriction map.
Design a strategy for manipulating DNA molecules in particular scenarios (eg. Radio labeling and sub cloning).
Distinguish the differences between prokaryotes and eukaryotes in terms of genome organization, gene structure, and expression and DNA replication.
Given a type of DNA damage explain the repair mechanisms that can occur.
Identify/recognize and explain the roles of the major proteins involved in the process of DNA replication/RNA synthesis and its regulation.
Distinguish the differences in DNA packaging/chromatin organization between prokaryotes and eukaryotes.
Predict the mRNA and the amino acid sequence given a DNA sequence.
Explain the contribution of DNA packaging/chromatin to gene expression and regulation.
Identify techniques to study DNA packaging/chromatin (eg CHIP, DNA sensitivity).
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